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guide•4 min read

How to reconstitute peptides: retatrutide and tirzepatide step by step

Reconstituting lyophilised peptides such as retatrutide and tirzepatide with bacteriostatic water: the steps in the lab, the concentration per vial and storage afterwards.

4 October 2026Redaktionen
How to reconstitute peptides: retatrutide and tirzepatide step by step

What you need

Step by step

  1. Prepare: let the peptide vial reach room temperature (about 15–20 minutes).
  2. Disinfect: wipe the rubber stopper of both the peptide vial and the water vial with an alcohol wipe and let it dry completely (at least 30 seconds).
  3. Draw the water: draw the required volume of bacteriostatic water, typically 1–2 ml, avoiding air bubbles.
  4. Add the water: insert the needle through the centre of the stopper and let the water run slowly down the inside wall of the vial, not directly onto the powder.
  5. Let it dissolve: leave the vial to stand; the powder dissolves on its own within a few minutes. Swirl gently if needed.
  6. Check: the solution should be clear and colourless. Discard it if it is cloudy or contains particles.

Never shake

Peptides are fragile molecules. Shaking can break down their structure. Swirl gently instead.

Concentration per vial

The concentration depends only on how much bacteriostatic water you add.

VialBAC waterConcentration
Retatrutide 10 mg1 ml10 mg/ml
Retatrutide 10 mg2 ml5 mg/ml
Retatrutide 10 mg3 mlabout 3.33 mg/ml
Tirzepatide 20 mg1 ml20 mg/ml
Tirzepatide 20 mg2 ml10 mg/ml

More water gives a more dilute solution, which makes small volumes easier to measure accurately in the lab.

Storage after reconstitution

  • Always in a fridge at 2–8 °C, away from light
  • Never freeze a reconstituted solution

More in the peptide storage guide, or read about the substances in the retatrutide guide and tirzepatide guide.

Our products are sold for research and laboratory use only and are not intended for humans or animals.

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